Land Invaders

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0:00:44 > 0:00:50Amphibians were the first backboned animals to leave the water

0:00:50 > 0:00:51and colonise the land.

0:00:51 > 0:00:55Today there are some 6,000 species of them,

0:00:55 > 0:00:58and new ones are constantly being discovered.

0:00:58 > 0:01:00We may not often see them,

0:01:00 > 0:01:03but during the breeding season, we certainly hear them.

0:01:03 > 0:01:05CROAKING AND CHIRRUPING

0:01:14 > 0:01:19Choruses like this ensure that we are well aware of frogs and toads.

0:01:26 > 0:01:29But there are other kinds of amphibians

0:01:29 > 0:01:31that don't make themselves so obvious.

0:01:31 > 0:01:36Newts and their close relatives, the salamanders.

0:01:37 > 0:01:41And even ones that have completely lost their legs.

0:01:43 > 0:01:47But all amphibians have one thing in common - a moist skin.

0:01:47 > 0:01:49If that dries, they die.

0:01:51 > 0:01:55And dealing with that danger dominates their lives.

0:01:55 > 0:01:58How are they to survive away from water?

0:02:06 > 0:02:09400 million years ago,

0:02:09 > 0:02:12the only backboned animals on the Earth were fish.

0:02:12 > 0:02:16The land was empty, except for insects and other invertebrates.

0:02:16 > 0:02:21But then, one of those fish managed to haul itself out of the water

0:02:21 > 0:02:23and up on to the land.

0:02:23 > 0:02:26You can see what sort of creature that might have been

0:02:26 > 0:02:29if you go to north-east Australia.

0:02:29 > 0:02:31There, the rivers only too often dry up.

0:02:34 > 0:02:39But one remarkable, ancient and extraordinary fish

0:02:39 > 0:02:41managed to survive,

0:02:41 > 0:02:44because it has a rare talent for a fish -

0:02:44 > 0:02:48it has lungs and can breathe air.

0:02:48 > 0:02:50And there's one at my feet, right here.

0:02:52 > 0:02:56Fossils just like it date from precisely the time

0:02:56 > 0:02:59when the great invasion of the land took place.

0:02:59 > 0:03:03On occasion, it rises to the surface and gulps air.

0:03:06 > 0:03:09The air goes into a pouch that opens from its throat,

0:03:09 > 0:03:12where the oxygen from it is absorbed.

0:03:12 > 0:03:15This is a lungfish.

0:03:18 > 0:03:21It punts itself along the river bottom,

0:03:21 > 0:03:26using two pairs of fleshy, muscular fins placed low on its body

0:03:26 > 0:03:28just like simple legs.

0:03:30 > 0:03:36Sometime around 360 million years ago, one of its remote ancestors

0:03:36 > 0:03:42used such limb-like fins to push itself up onto the land.

0:03:47 > 0:03:52That pioneer may have looked much like this strange monster

0:03:52 > 0:03:55that haunts the waterways of Japan.

0:03:58 > 0:04:02It's the giant salamander, the biggest of all living amphibians,

0:04:02 > 0:04:06that grows to a metre or more in length.

0:04:11 > 0:04:15It, too, has lungs and breathes air.

0:04:15 > 0:04:19But even so, it almost never leaves the water.

0:04:21 > 0:04:25Males make their dens in both natural and man-made retreats

0:04:25 > 0:04:26in the riverbanks,

0:04:26 > 0:04:29and defend them against all other males.

0:04:30 > 0:04:35A newcomer arrives, looking for a breeding den of his own.

0:05:10 > 0:05:11It won't be here.

0:05:18 > 0:05:22The resident male has good reason to be so defensive.

0:05:25 > 0:05:27He's guarding a batch of eggs,

0:05:27 > 0:05:32left by a female who visited him a few days earlier.

0:05:32 > 0:05:36Like fish eggs, amphibian eggs have no protective shell.

0:05:36 > 0:05:38They can only develop in moisture of some kind,

0:05:38 > 0:05:43and amphibians, no matter where they live, must find ways to provide it.

0:05:46 > 0:05:50The alpine newt lives on land for about half the year,

0:05:50 > 0:05:52hunting for slugs and worms.

0:05:54 > 0:05:57In winter, they lie dormant beneath the snow,

0:05:57 > 0:06:00but come the spring, they get the urge to breed.

0:06:05 > 0:06:09A female is swollen with eggs and needs to lay,

0:06:09 > 0:06:12so she has to go back to water.

0:06:18 > 0:06:22And there, a male is awaiting her.

0:06:22 > 0:06:26He has already developed his breeding colours

0:06:26 > 0:06:29and knows how to flaunt them to impress her.

0:06:34 > 0:06:36He wafts a pheromone, a sexual stimulant,

0:06:36 > 0:06:39towards her with beats of his tail.

0:06:52 > 0:06:55She senses it through her nostrils.

0:07:05 > 0:07:08She tastes it in her mouth.

0:07:14 > 0:07:19Having caught her interest, he turns and moves away from her.

0:07:21 > 0:07:23His genital opening is greatly swollen,

0:07:23 > 0:07:26and from it comes a small white capsule.

0:07:26 > 0:07:29It's a packet of sperm.

0:07:32 > 0:07:38The female, led by the male, walks directly over it.

0:07:38 > 0:07:41He stops and so does she,

0:07:41 > 0:07:45with her genital opening exactly above the sperm packet,

0:07:45 > 0:07:47and she picks it up.

0:07:53 > 0:07:58So, as in many fish, mating occurs with little or no physical contact

0:07:58 > 0:08:01between the two partners.

0:08:03 > 0:08:07Two or three days later, she begins to lay.

0:08:12 > 0:08:16Each of her eggs is deposited individually.

0:08:27 > 0:08:31As an egg emerges, she wraps the leaf around it with her hind legs,

0:08:31 > 0:08:34and then holds it there while the edges bond.

0:08:36 > 0:08:40She will lay several eggs a day for week after week,

0:08:40 > 0:08:44until, eventually, she may have produced several hundred.

0:08:49 > 0:08:52But all this has to be done in water.

0:08:52 > 0:08:57She has still not broken her link with her fishy ancestry.

0:08:59 > 0:09:03In North America, in the eastern half of the country,

0:09:03 > 0:09:07there are many kinds of small salamanders, only a few inches long,

0:09:07 > 0:09:12that have taken one further step away from the aquatic life.

0:09:14 > 0:09:19In spring, the woodlands are drenched in rain,

0:09:19 > 0:09:20and suddenly, in response,

0:09:20 > 0:09:23an amphibian army appears among the leaf litter.

0:09:26 > 0:09:28Marbled salamanders.

0:09:31 > 0:09:34First to emerge are the males.

0:09:34 > 0:09:36They're in search of females.

0:09:37 > 0:09:40They have spent the winter deep in the damp leaf litter,

0:09:40 > 0:09:45breathing by absorbing oxygen from the air, through their moist skins.

0:09:45 > 0:09:48For them, the land is truly home.

0:09:48 > 0:09:51If they were submerged in water for any length of time,

0:09:51 > 0:09:54they might well drown.

0:09:55 > 0:09:59Nonetheless, their courtship techniques are much the same

0:09:59 > 0:10:02as those used in water by newts.

0:10:06 > 0:10:10The males produce pheromones that excite the females.

0:10:17 > 0:10:22They deposit capsules of sperm on the damp ground.

0:10:22 > 0:10:27And the females crawl over them and take them in.

0:10:28 > 0:10:33In due course, each female lays her soft-skinned eggs on the ground,

0:10:33 > 0:10:36and stays beside them, on guard.

0:10:37 > 0:10:41Here, it's damp enough to prevent her eggs from drying,

0:10:41 > 0:10:44and they're already developing rapidly.

0:10:50 > 0:10:55Eventually, the continuing rains flood the woodland floor.

0:10:57 > 0:11:01But now the female's needs and those of her eggs are exactly opposite.

0:11:01 > 0:11:05They will need water in order to breathe,

0:11:05 > 0:11:08but she could drown in it, so she has to leave.

0:11:13 > 0:11:17The young inside their capsules are developing into creatures

0:11:17 > 0:11:20fundamentally different from their parents,

0:11:20 > 0:11:23a form that is characteristic of amphibians.

0:11:23 > 0:11:25They are becoming tadpoles.

0:11:27 > 0:11:31They swim free, equipped with feathery gills that enable them

0:11:31 > 0:11:33to extract oxygen from the water.

0:11:33 > 0:11:36They are truly aquatic creatures...

0:11:38 > 0:11:42..but they have front legs as well as gills.

0:11:49 > 0:11:53And within days, they develop back legs as well.

0:11:56 > 0:11:58As time passes, they grow stronger.

0:11:58 > 0:12:01Their gills wither and disappear,

0:12:01 > 0:12:04and at last, they're miniature versions of their parents,

0:12:04 > 0:12:07and are ready to leave the water forever,

0:12:07 > 0:12:10and to start on their land-living lives.

0:12:10 > 0:12:15But what tempted those ancient fish to leave the water

0:12:15 > 0:12:17in the first place?

0:12:17 > 0:12:19Food.

0:12:20 > 0:12:23When the first amphibians moved out of water,

0:12:23 > 0:12:26the land was already swarming with insects.

0:12:26 > 0:12:30And the amphibians have evolved a special weapon

0:12:30 > 0:12:31with which to catch them.

0:12:39 > 0:12:43Salamanders, however, have not yet developed the athleticism

0:12:43 > 0:12:47needed for a high-speed chase and a lightning pounce.

0:12:47 > 0:12:51Their hunts are rather solemn, sedate affairs.

0:13:12 > 0:13:16A simple contraction of the muscles surrounding the tongue

0:13:16 > 0:13:18is all that's needed to shoot it forward.

0:13:23 > 0:13:27Some salamanders have a tongue that is about three-quarters

0:13:27 > 0:13:30the length of their body, but most species

0:13:30 > 0:13:33have to get pretty close to their prey if they're to catch it.

0:13:40 > 0:13:44Although the adult marbled salamander lives entirely on land,

0:13:44 > 0:13:48it nonetheless needed water at the very beginning of its life.

0:13:48 > 0:13:52But there are other species of salamander in North America

0:13:52 > 0:13:54that have managed to break even that link

0:13:54 > 0:13:56with their distant aquatic past.

0:14:03 > 0:14:06This is a gold mine.

0:14:06 > 0:14:08The people who dug it found nothing.

0:14:08 > 0:14:11But biologists, who came later,

0:14:11 > 0:14:14found gold of their own special kind.

0:14:23 > 0:14:27They discovered a colony of a species called the slimy salamander,

0:14:27 > 0:14:30that could be properly observed throughout the summer,

0:14:30 > 0:14:32when normally they're hidden in the leaf litter.

0:14:32 > 0:14:34They were all females,

0:14:34 > 0:14:38and their behaviour proved to be very surprising indeed.

0:14:43 > 0:14:49These salamanders come down in early summer, in about June,

0:14:49 > 0:14:55and will travel several hundred metres down along this mineshaft

0:14:55 > 0:14:59to exactly the same ledge, within an inch or so,

0:14:59 > 0:15:02that they used the previous year.

0:15:02 > 0:15:08And they have been seen doing that for at least five or six years.

0:15:08 > 0:15:10And they don't eat.

0:15:10 > 0:15:13They will stay down here for six or seven months,

0:15:13 > 0:15:16sustained only by the food reserves

0:15:16 > 0:15:20that they've accumulated in their fat tails.

0:15:22 > 0:15:25Down here there is permanent moisture,

0:15:25 > 0:15:27however hot and dry it gets outside.

0:15:30 > 0:15:33The salamanders clearly prefer to cluster together,

0:15:33 > 0:15:35close to one another,

0:15:35 > 0:15:39for the rock walls of the mineshaft elsewhere are totally uninhabited.

0:15:41 > 0:15:45However, this open-plan way of life,

0:15:45 > 0:15:47while it's clearly very successful,

0:15:47 > 0:15:50nonetheless comes at a price.

0:15:51 > 0:15:55Some of the females here are up to no good.

0:15:55 > 0:15:58They failed to fatten up enough during the spring,

0:15:58 > 0:16:03and they're hungry and in search of a good meal.

0:16:03 > 0:16:08And the eggs and young of their other salamanders

0:16:08 > 0:16:10will do very well.

0:16:11 > 0:16:14To see exactly what these creatures are doing,

0:16:14 > 0:16:19we need to turn off our torches and turn on the infrared camera.

0:16:27 > 0:16:30Here comes one of those marauding females.

0:16:40 > 0:16:45She must have located this mother, guarding her eggs, by smell,

0:16:45 > 0:16:48for all this is going on in total darkness.

0:17:07 > 0:17:09So some amphibians, when needs be,

0:17:09 > 0:17:15are neither sluggish, insensitive nor lacking in maternal concern.

0:17:20 > 0:17:22And mother wins the day.

0:17:26 > 0:17:28The salamanders' need to keep moist

0:17:28 > 0:17:31means that they seldom come out into the open,

0:17:31 > 0:17:35but find their prey by pushing through the leaf litter.

0:17:35 > 0:17:38And to do that, it helps to be slim.

0:17:38 > 0:17:40Very slim.

0:17:40 > 0:17:43Legs are less in the way if they're small.

0:17:46 > 0:17:50And one great group of burrowing amphibians

0:17:50 > 0:17:52has lost its legs altogether.

0:17:57 > 0:18:01You might think that this was a giant earthworm.

0:18:01 > 0:18:03But if you picked it up,

0:18:03 > 0:18:07you would immediately realise it's got a strong, firm backbone.

0:18:07 > 0:18:09It's a caecilian.

0:18:10 > 0:18:15Caecilians are found in almost all rainforests.

0:18:15 > 0:18:17But they are seldom seen,

0:18:17 > 0:18:20for they spend nearly all their lives underground.

0:18:21 > 0:18:24The female, having produced her young,

0:18:24 > 0:18:28stays in her nest chamber to protect them.

0:18:34 > 0:18:36Caecilian eyes are rudimentary.

0:18:36 > 0:18:38They're covered in skin, and scarcely function.

0:18:38 > 0:18:41In the darkness underground, however,

0:18:41 > 0:18:43the animals have no need for them.

0:18:46 > 0:18:50The young enthusiastically lick a secretion from a gland

0:18:50 > 0:18:52at the end of their mother's tail,

0:18:52 > 0:18:55and their constant hunger seems to be the factor

0:18:55 > 0:18:58that keeps this little blind family together.

0:19:06 > 0:19:07In a single week,

0:19:07 > 0:19:12the young, incredibly, increase their weight by ten times,

0:19:12 > 0:19:15apparently just from drinking her secretion.

0:19:15 > 0:19:18But could that be their only food?

0:19:19 > 0:19:22As we filmed, one of the youngsters

0:19:22 > 0:19:25revealed a clue to their rapid growth.

0:19:25 > 0:19:26It yawned.

0:19:28 > 0:19:32It already had hook teeth like a baby shark.

0:19:32 > 0:19:37It surely doesn't need these if it's going to do nothing but drinking.

0:19:37 > 0:19:39Could it be feeding on something else?

0:19:43 > 0:19:48A few hours later, our cameras, for the first time, revealed the answer.

0:19:50 > 0:19:52There was a sudden frenzy of activity.

0:19:52 > 0:19:55The babies started swarming all over their mother.

0:20:07 > 0:20:10They were tearing at her flanks,

0:20:10 > 0:20:12ripping off segments of her skin -

0:20:12 > 0:20:15skin that proved to be full of fat.

0:20:25 > 0:20:30It turned out that she regrew her skin every three days,

0:20:30 > 0:20:33to provide her young with another nourishing meal.

0:20:44 > 0:20:48Blind, elongated and legless caecilians may be,

0:20:48 > 0:20:52but simple, inoffensive earthworms they are not.

0:20:54 > 0:20:59The most numerous and successful of all amphibians, however,

0:20:59 > 0:21:03have kept their legs and developed them spectacularly.

0:21:27 > 0:21:30Some are walkers.

0:21:37 > 0:21:40Others are climbers.

0:21:49 > 0:21:51There are hoppers.

0:21:56 > 0:21:58There are even gliders,

0:21:58 > 0:22:02who use the membranes on their feet like parachutes.

0:22:18 > 0:22:23If their skin is very moist, we call these creatures frogs.

0:22:23 > 0:22:27If it's less so, we call them toads,

0:22:27 > 0:22:29but they all belong to the same group.

0:22:37 > 0:22:42There are some 5,500 different kinds of frogs and toads

0:22:42 > 0:22:45in the world today,

0:22:45 > 0:22:49and here, in the leaf litter in this Madagascan forest,

0:22:49 > 0:22:52is the tiniest of them all.

0:22:52 > 0:22:55This is fully adult,

0:22:55 > 0:22:57and in its tiny body,

0:22:57 > 0:23:00which is only a centimetre long,

0:23:00 > 0:23:02is packed a beating heart,

0:23:02 > 0:23:06a skeleton, a gut, a brain.

0:23:07 > 0:23:10It's a miracle of miniaturisation.

0:23:12 > 0:23:14And this basic body plan

0:23:14 > 0:23:17not only comes in all sizes,

0:23:17 > 0:23:19but many different shapes,

0:23:19 > 0:23:21which has enabled frogs and toads

0:23:21 > 0:23:25to colonise all kinds of different environments.

0:23:27 > 0:23:34Out of water, frogs found a new way to communicate with one another.

0:23:34 > 0:23:36CROAKING

0:23:46 > 0:23:50Amphibian lungs are comparatively feeble,

0:23:50 > 0:23:54so frogs amplify their calls with cheek or throat pouches

0:23:54 > 0:23:55which act as resonators.

0:23:58 > 0:24:01The call of a frog in this South African pool

0:24:01 > 0:24:04can be heard over a mile away.

0:24:07 > 0:24:12It's the painted reed frog, the loudest caller of all, for his size.

0:24:25 > 0:24:30But a female is not only impressed by the loudness of a male's call.

0:24:33 > 0:24:34She also judges him

0:24:34 > 0:24:38by how frequently he manages to make that call.

0:24:50 > 0:24:53Calling is a very demanding activity,

0:24:53 > 0:24:56requiring a male to increase his energy consumption

0:24:56 > 0:24:58by about 20 times,

0:24:58 > 0:25:01so in picking the loudest and fastest caller,

0:25:01 > 0:25:04the female is also selecting the fittest and most vigorous male

0:25:04 > 0:25:07as the father of her offspring.

0:25:16 > 0:25:18He's the one.

0:25:26 > 0:25:29Success, and silence, for a few minutes.

0:25:35 > 0:25:37In some circumstances, however,

0:25:37 > 0:25:40calls need reinforcing with gestures.

0:25:45 > 0:25:51The sound of rushing water could drown out the calls of a frog.

0:25:51 > 0:25:54However, here in this stream in Panama,

0:25:54 > 0:25:57there's a species living alongside

0:25:57 > 0:26:00that has developed a novel way of dealing with that problem.

0:26:02 > 0:26:05The rare and wonderful golden frog.

0:26:05 > 0:26:08It does have a voice, but it's not loud.

0:26:16 > 0:26:20Individual males set up their territories beside the river,

0:26:20 > 0:26:23and then wait for females to turn up.

0:26:23 > 0:26:28And since good positions for a territory are not common,

0:26:28 > 0:26:31they may have to hold them against intruders.

0:26:32 > 0:26:34And here one comes.

0:26:36 > 0:26:39Just in case his call is inaudible,

0:26:39 > 0:26:42he makes his message clear with a wave.

0:26:48 > 0:26:51And his rival waves back.

0:26:53 > 0:26:56He repeats his message so there's no misunderstanding.

0:27:08 > 0:27:10But rival is not deterred.

0:27:11 > 0:27:14Well, that makes things perfectly clear.

0:27:17 > 0:27:19Another arrives.

0:27:19 > 0:27:23Perhaps, at last, this is a female.

0:27:37 > 0:27:41No, it's another male, so there will have to be a wrestling match.

0:27:46 > 0:27:49That should teach him.

0:27:50 > 0:27:55His rival signals submission by keeping his head down.

0:28:21 > 0:28:25Now where are those females?

0:28:28 > 0:28:29And here she is.

0:28:31 > 0:28:35She is pure, unblemished gold and much bigger than he is.

0:28:45 > 0:28:48While he is fully occupied, another challenger arrives.

0:28:53 > 0:28:56Since he's already in position,

0:28:56 > 0:28:59there's no point in breaking away for another wrestling match,

0:28:59 > 0:29:01so he hangs on.

0:29:07 > 0:29:11The golden frog has a powerful poison in its skin,

0:29:11 > 0:29:13so it can afford to be conspicuous,

0:29:13 > 0:29:16but most frogs find safety in camouflage.

0:29:20 > 0:29:25This is a South American red-eyed tree frog,

0:29:25 > 0:29:29a close match for the leaves on which it habitually sits.

0:29:32 > 0:29:36The eggs are not very conspicuous either,

0:29:36 > 0:29:39just little blobs in transparent jelly.

0:29:39 > 0:29:42And they're always laid over water.

0:29:46 > 0:29:49They develop very rapidly.

0:30:09 > 0:30:14In less than a week, they've become recognisable tadpoles,

0:30:14 > 0:30:16almost ready for freedom.

0:30:16 > 0:30:21Then the jelly liquefies and they simply drop into the water beneath.

0:30:23 > 0:30:27But some don't survive long enough to do so.

0:30:31 > 0:30:33Wasps raid the cluster

0:30:33 > 0:30:37and carry off the unhatched tadpoles to feed their young.

0:31:07 > 0:31:10But the tadpoles are not entirely helpless.

0:31:12 > 0:31:16By the time they're five days old, they know when they're under attack,

0:31:16 > 0:31:19and, what's more, they can do something about it.

0:31:21 > 0:31:23There.

0:31:24 > 0:31:28Quick wriggle and the tadpole drops to safety.

0:31:36 > 0:31:40The alarm spreads quickly through the whole cluster,

0:31:40 > 0:31:41and they all take a dive.

0:31:53 > 0:31:56Their tails are not yet fully developed,

0:31:56 > 0:31:59but they can swim well enough to take refuge

0:31:59 > 0:32:01beneath the leaves of the water plants.

0:32:24 > 0:32:28So if there's a choice between being carried off by a wasp

0:32:28 > 0:32:32and taking an early bath, there's no competition.

0:32:33 > 0:32:37But not all frogs abandon their young.

0:32:37 > 0:32:40If you're big enough, you can stay and defend them,

0:32:40 > 0:32:44and the male giant African bullfrog is as big as a football.

0:32:46 > 0:32:49His pool, which formed during the rainy season,

0:32:49 > 0:32:52lies near the margin of a much bigger pond.

0:32:56 > 0:32:58The nursery pool was a good place to lay,

0:32:58 > 0:33:00for it had none of the predators

0:33:00 > 0:33:03that abound in the bigger, permanent pond.

0:33:03 > 0:33:06But as the dry season warms up,

0:33:06 > 0:33:09that smaller pool begins to evaporate.

0:33:11 > 0:33:14Tadpoles are now in real danger.

0:33:16 > 0:33:18Father takes action.

0:33:21 > 0:33:23He starts to dig a canal

0:33:23 > 0:33:27to enable his endangered tadpoles to reach the deeper pond nearby.

0:33:36 > 0:33:40It will be touch and go, but if they can only get to the bigger pond,

0:33:40 > 0:33:45they're now vigorous enough to have a reasonable chance of survival.

0:33:51 > 0:33:53Breakthrough.

0:34:01 > 0:34:04And Father leads the way.

0:34:17 > 0:34:19In the rainforests of South America,

0:34:19 > 0:34:23the daily rains create a multitude of tiny pools

0:34:23 > 0:34:26in the centre of many plants.

0:34:26 > 0:34:32This tiny poison arrow frog is carrying his tadpole piggy-back.

0:34:32 > 0:34:37It hatched on a leaf and now he's taking it to a pool in a bromeliad,

0:34:37 > 0:34:39high up in the branches.

0:34:39 > 0:34:41The tadpole wriggles off.

0:34:43 > 0:34:45He may have half a dozen babies,

0:34:45 > 0:34:49each of which he puts into its own tiny pool.

0:34:56 > 0:34:59He makes regular tours of all his nurseries,

0:34:59 > 0:35:03checking on his tadpoles' welfare.

0:35:09 > 0:35:12This youngster is hungry,

0:35:12 > 0:35:17and tells him so by nibbling his legs and vibrating against his body.

0:35:21 > 0:35:27But the male can't feed the tadpole himself - he needs help.

0:35:37 > 0:35:39He has to find a female.

0:35:47 > 0:35:50There she is.

0:35:54 > 0:35:56He calls.

0:36:05 > 0:36:09And she follows.

0:36:15 > 0:36:17He has to lead,

0:36:17 > 0:36:21for only he knows exactly where he deposited each tadpole.

0:36:23 > 0:36:26This one is now very hungry indeed.

0:36:30 > 0:36:33He calls to the female encouragingly.

0:36:38 > 0:36:43She jumps in, perhaps to assess the situation.

0:36:46 > 0:36:47Out she comes,

0:36:47 > 0:36:51without having done what's required, so he keeps calling.

0:36:51 > 0:36:55In she goes a second time.

0:36:55 > 0:36:59This time she produces food for the hungry tadpole,

0:36:59 > 0:37:01an infertile egg.

0:37:06 > 0:37:07There.

0:37:11 > 0:37:16Out she comes, and mother and father embrace.

0:37:28 > 0:37:32Baby has its dinner.

0:37:42 > 0:37:47Australia, in the south-east, has temperate rainforests.

0:37:48 > 0:37:52A cluster of frogs' eggs on the damp ground.

0:37:52 > 0:37:56When these hatch, the tadpoles will also need a moist nursery.

0:37:56 > 0:38:01Father, a marsupial frog, is on guard.

0:38:01 > 0:38:04The eggs are developing fast.

0:38:09 > 0:38:11The male has to keep a careful eye on them,

0:38:11 > 0:38:16for he must be close beside them at the very moment when they hatch.

0:38:24 > 0:38:26It's going to be a long wait.

0:38:26 > 0:38:29At least 11 days.

0:38:39 > 0:38:43He seems to have decided that the crucial moment has arrived,

0:38:43 > 0:38:47and lowers himself onto the eggs.

0:38:52 > 0:38:55As he does so, the tough egg membranes liquefy,

0:38:55 > 0:38:57and the young wriggle free.

0:38:58 > 0:39:02He has two pouches in his skin, one on each hip,

0:39:02 > 0:39:06and the tadpoles start to squirm into them.

0:39:15 > 0:39:18Competition between the tadpoles is intense,

0:39:18 > 0:39:22for there are more of them than he can accommodate in his pouches.

0:39:47 > 0:39:52At last, he's taken on board as many as he can manage.

0:39:52 > 0:39:57He will now look after them for up to six weeks.

0:40:01 > 0:40:05The young remain in his pouches continuing their development,

0:40:05 > 0:40:08fuelled by the remains of the yolk in their infant stomachs.

0:40:14 > 0:40:18And then, one night, his behaviour changes.

0:40:20 > 0:40:22His flanks are rippling.

0:40:31 > 0:40:35The first of his young is emerging.

0:41:09 > 0:41:12The profound transformation

0:41:12 > 0:41:15that converted a tadpole into this young frog

0:41:15 > 0:41:20took place entirely within its father's moist pouch.

0:41:28 > 0:41:31The parched bush country of southern Africa.

0:41:31 > 0:41:37Here, it rains only twice a year, and then only briefly.

0:41:39 > 0:41:45But when it does, the ground, in places, erupts.

0:41:52 > 0:41:55Rain frogs, as they're aptly called,

0:41:55 > 0:41:59have been waiting for months below ground for this moment.

0:42:03 > 0:42:06After starving for so long, they're keen to feed.

0:42:38 > 0:42:43As darkness falls, the males begin to call.

0:42:43 > 0:42:45HIGH-PITCHED CHIRRUP

0:42:57 > 0:43:01Females are fat with eggs.

0:43:08 > 0:43:13The males are so much smaller that they can't embrace a female,

0:43:13 > 0:43:16so they produce glue from glands on their underside,

0:43:16 > 0:43:19and stick themselves to their partner's back.

0:43:22 > 0:43:26But sometimes, that only results in a chain of enthusiastic

0:43:26 > 0:43:29but undiscriminating males, stuck to one another.

0:43:37 > 0:43:40Their brief time above ground has come to an end.

0:43:40 > 0:43:43The female starts to dig.

0:43:44 > 0:43:48The diminutive male, being stuck on, goes with her.

0:43:48 > 0:43:52He will fertilise the eggs later, below ground.

0:43:59 > 0:44:03Her stay on the surface is over.

0:44:06 > 0:44:11The female has excavated a little chamber for herself,

0:44:11 > 0:44:13and below that she's made a second one,

0:44:13 > 0:44:16which she's filled with a frothy foam.

0:44:19 > 0:44:23This is the nursery for her tadpoles.

0:44:43 > 0:44:48The female stays underground, away from the lethal heat,

0:44:48 > 0:44:49for several more weeks.

0:44:59 > 0:45:05By now, her offspring have almost completed their time as tadpoles.

0:45:15 > 0:45:18The rains return.

0:45:23 > 0:45:27Below ground, the youngsters await their release.

0:45:46 > 0:45:49The female leads the way.

0:45:49 > 0:45:53And her brood are with her.

0:46:22 > 0:46:25Rain is even rarer in Australia.

0:46:25 > 0:46:29There, in the central deserts, it may not fall for years on end.

0:46:32 > 0:46:35But there are amphibians even here.

0:46:35 > 0:46:38Little toads that remain underground,

0:46:38 > 0:46:42in a state of suspended animation, for years,

0:46:42 > 0:46:45just to take advantage of a few rainy days.

0:46:47 > 0:46:52After the rains have fallen, spadefoot toads all emerge together.

0:46:52 > 0:46:57They must feed and breed, if possible, before the sun rises.

0:47:03 > 0:47:06But the desert dries only too quickly,

0:47:06 > 0:47:09even after the heaviest of storms.

0:47:09 > 0:47:13Temperatures rise to 50 degrees centigrade.

0:47:13 > 0:47:15Now water will evaporate instantly.

0:47:15 > 0:47:18This is one of the hottest places on Earth.

0:47:21 > 0:47:25So the toads have to retreat, once again, below ground.

0:47:25 > 0:47:29The miracle is that they're here at all.

0:47:32 > 0:47:36A toad that can live in as parched a desert as this

0:47:36 > 0:47:41is impressive evidence of the versatility of the amphibians,

0:47:41 > 0:47:45the way they can adapt their behaviour and their anatomy

0:47:45 > 0:47:47to live so far away from water.

0:47:47 > 0:47:49But there's one group of animals

0:47:49 > 0:47:54that can really call the desert their own - the lizards.

0:47:54 > 0:47:59And we'll look at them in the next episode of Life In Cold Blood.

0:48:08 > 0:48:12Amphibians are the most threatened group of vertebrates on the planet.

0:48:12 > 0:48:14In recent years,

0:48:14 > 0:48:19a strange and lethal fungal disease has started to spread among them.

0:48:21 > 0:48:25The golden frog, which lives only in one small area in Panama,

0:48:25 > 0:48:27was in particular danger,

0:48:27 > 0:48:30as the disease is already on the frontier of its territory.

0:48:30 > 0:48:33If we were to film it at all, we would have to move quickly.

0:48:36 > 0:48:39For series producer Miles Barton,

0:48:39 > 0:48:42that meant cutting short Christmas.

0:48:54 > 0:48:56We had been told that in Panama,

0:48:56 > 0:48:59the frogs' few remaining breeding streams

0:48:59 > 0:49:03were being rapidly destroyed by the building of a new road,

0:49:03 > 0:49:07making the last tiny population even more at risk from the disease.

0:49:10 > 0:49:13The fungus clogs the animal's moist skin.

0:49:13 > 0:49:16Since all frogs breathe through their skin,

0:49:16 > 0:49:19infected animals die from suffocation.

0:49:21 > 0:49:24Frog biologist Erik Lindquist,

0:49:24 > 0:49:27who first described the golden frog's signalling behaviour,

0:49:27 > 0:49:30helped the film team to thoroughly disinfect their kit

0:49:30 > 0:49:33before travelling into the frogs' territory.

0:49:36 > 0:49:39Freshly scrubbed up, Erik took the team

0:49:39 > 0:49:42to one of the golden frog's last-known breeding sites.

0:49:47 > 0:49:50But would they still be there?

0:49:50 > 0:49:52DISTANT CROAK

0:49:52 > 0:49:54Yeah, you hear that? That's a male calling.

0:49:59 > 0:50:02We have another male crawling up over here,

0:50:02 > 0:50:04crawling up the rock face.

0:50:05 > 0:50:09But with the fungus approaching at a rate of up to 25 miles a year,

0:50:09 > 0:50:14the frogs were rapidly disappearing from all their known breeding sites.

0:50:14 > 0:50:17The advance crew immediately set about filming

0:50:17 > 0:50:20as much of the behaviour as they could.

0:50:28 > 0:50:30By the time I arrived,

0:50:30 > 0:50:34there was only one remaining location where the frogs survived.

0:50:40 > 0:50:43Where exactly are we going?

0:50:43 > 0:50:46I would prefer not saying, precisely.

0:50:46 > 0:50:49You see, this is really the last population of the golden frog

0:50:49 > 0:50:51left in the wild,

0:50:51 > 0:50:55and historically, the locals have been collecting out these animals

0:50:55 > 0:51:02as good luck talisman, and so now we're left with just one population.

0:51:02 > 0:51:08I'm concerned that if the secret locality gets given out,

0:51:08 > 0:51:12- there'll be international collectors that would come.- Really?

0:51:12 > 0:51:14Sure. They're rare enough now

0:51:14 > 0:51:20where many people would pay top dollar for these animals.

0:51:20 > 0:51:23Were they ever what you might call common?

0:51:23 > 0:51:25When I talk to people who have been here in the past,

0:51:25 > 0:51:30the populations were so abundant that one would have to watch where they're

0:51:30 > 0:51:32stepping to keep from killing one.

0:51:32 > 0:51:33- Really?- Yes, yes.

0:51:35 > 0:51:38Erik has his own low-tech method of finding them,

0:51:38 > 0:51:40which he assures me normally works.

0:51:44 > 0:51:47LOW WHISTLING

0:51:50 > 0:51:53See, when you call, sometimes they'll call back

0:51:53 > 0:51:56and they'll reveal their locations.

0:51:56 > 0:51:58Sometimes they're tucked away behind leaves

0:51:58 > 0:52:00and they're really difficult to find.

0:52:00 > 0:52:02Hopefully we can elicit a response.

0:52:02 > 0:52:05HE WHISTLES

0:52:08 > 0:52:11It's the fastest way to get them to shut up.

0:52:15 > 0:52:16- Was that him?- Yeah, listen.

0:52:20 > 0:52:22- So they're here?- They're here.

0:52:22 > 0:52:24There's one over there.

0:52:29 > 0:52:33See him right there. Looks like a male.

0:52:33 > 0:52:34Make him do it again.

0:52:34 > 0:52:37HE WHISTLES

0:52:41 > 0:52:43FROG WHISTLES

0:52:44 > 0:52:47- You have to hum and whistle at the same time.- Can't do it!

0:52:47 > 0:52:49See if he can.

0:52:52 > 0:52:54HE TRIES TO WHISTLE

0:53:00 > 0:53:04Now we knew the frogs were still here, we could complete the filming.

0:53:06 > 0:53:09The local people have always treasured

0:53:09 > 0:53:11their remarkable little frog,

0:53:11 > 0:53:15but Erik was the first to document its signalling behaviour.

0:53:15 > 0:53:17It was an animal that was just walking.

0:53:17 > 0:53:20I wasn't sure if the animal was trying to flush out prey

0:53:20 > 0:53:24or if it was using it in a communication role.

0:53:24 > 0:53:26And so a group of us set out

0:53:26 > 0:53:29to look at whether or not this was communication.

0:53:29 > 0:53:32We tried mirror presentations to the animals.

0:53:32 > 0:53:34When you presented them with a mirror,

0:53:34 > 0:53:36they would hand-wave at the mirror

0:53:36 > 0:53:40as opposed to, say, the backside of a mirror that wasn't reflective.

0:53:41 > 0:53:45Some of us have looked specifically at an LCD screen,

0:53:45 > 0:53:50a little television with a hand-waving, semaphoring frog,

0:53:50 > 0:53:55and it has elicited a number of responses, specifically from males.

0:53:55 > 0:53:59What, you show a television picture to a male and he waves back?

0:53:59 > 0:54:03He waves back and he'll even call, to the male on the television screen.

0:54:03 > 0:54:05- Really?- It's really fascinating.

0:54:06 > 0:54:10They then experimented with a life-size plastic model,

0:54:10 > 0:54:12complete with waving arm,

0:54:12 > 0:54:17the sort of high-tech gear I thought I might manage to operate myself.

0:54:32 > 0:54:35It's not as easy as you might think.

0:54:40 > 0:54:42Erik showed me how it should be done.

0:54:42 > 0:54:47You've got to get that slow-motion wave just right.

0:54:49 > 0:54:52The frogs waved.

0:55:01 > 0:55:03They called.

0:55:05 > 0:55:08They even attacked.

0:55:08 > 0:55:12So that wave really is a form of communication.

0:55:12 > 0:55:16So they're just saying, "Keep off, keep off."

0:55:16 > 0:55:17Huh, is that right?

0:55:17 > 0:55:21We're not sure. Sometimes there seem to be certain hand waves

0:55:21 > 0:55:23that may indicate appeasement,

0:55:23 > 0:55:26showing that, "I'm just walking through, perhaps, your territory.

0:55:26 > 0:55:28"Don't bother me."

0:55:28 > 0:55:30Really? "Ah, please."

0:55:32 > 0:55:35But how endangered is the golden frog?

0:55:36 > 0:55:39This is it. What you see.

0:55:39 > 0:55:44You're going to be the last crew to film these in the wild.

0:55:45 > 0:55:47'And indeed we were.

0:55:47 > 0:55:49'Soon after finishing filming,

0:55:49 > 0:55:52'the local scientists decided the time had come

0:55:52 > 0:55:56'to take all the surviving golden frogs into captivity,

0:55:56 > 0:55:59'before the fungus arrives here and kills them all.

0:55:59 > 0:56:02'They and other rare species of frog also threatened

0:56:02 > 0:56:06'were being brought back to a special frog hospital,

0:56:06 > 0:56:08'where I was introduced to some of the other patients.'

0:56:08 > 0:56:10So what are these?

0:56:10 > 0:56:12They're nocturnal, so they spend...

0:56:12 > 0:56:15'Here, they're being treated daily with a fungicide.

0:56:15 > 0:56:17'But without a vaccine to protect them,

0:56:17 > 0:56:20'and with the fungus still at large in the forest,

0:56:20 > 0:56:23'they can't be re-introduced into their proper home.'

0:56:28 > 0:56:31Frogs, so common in these humid forests,

0:56:31 > 0:56:33are crucial links in the ecology.

0:56:33 > 0:56:37If they disappear, all kinds of food chains will be broken.

0:56:37 > 0:56:41The effect could be little short of catastrophic to wildlife in general.

0:56:44 > 0:56:45And sadly, for now at least,

0:56:45 > 0:56:50it seems that the golden frog has waved its last in the wild.

0:57:15 > 0:57:18Subtitles by Red Bee Media Ltd

0:57:29 > 0:57:32E-mail subtitling@bbc.co.uk